Genotyping and primer details


Genotyping and primer details for 361C10 - At1g64210
Line ID 361C10
Locus At1g64210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:23832635 of line 361C10 Chr1:23832635
   
Insertion allele amplicon 8474 - EQ36
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EQ36: 5'-GGGTGCTTTTGGTACAACCTATAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:23831977
   
Insertion allele amplicon 8474 - KF47
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KF47: 5'-GCATGAACTCAGATCCTAAACCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:23833140
   
wt amplicon (over InsPos) KF47 - EQ36
wt amplicon start Chr1:23831977
wt amplicon end Chr1:23833140
wt amplicon size 1164 bp
Locus specific primer EQ36: 5'-GGGTGCTTTTGGTACAACCTATAA-3'
2nd locus specific primer KF47: 5'-GCATGAACTCAGATCCTAAACCTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11