Genotyping and primer details


Genotyping and primer details for 309E09 - At1g66410
Line ID 309E09
Locus At1g66410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:24774963 of line 309E09 Chr1:24774963
   
Insertion allele amplicon 8474 - FN65
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FN65: 5'-TAAGAACTCAGGGAAGTCAATGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:24774671
   
Insertion allele amplicon 8474 - RC01
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer RC01: 5'-TGATTGAATCGTTTGATTGTCTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:24775491
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11