Genotyping and primer details


Genotyping and primer details for 126H05 - At1g67980
Line ID 126H05
Locus At1g67980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:25488501 of line 126H05 Chr1:25488501
   
Insertion allele amplicon 3144 - 60wr
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 60wr: 5'-AAAACTTTGGTCATATCATTTGGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:25489398
   
Insertion allele amplicon 8474 - LD16
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer LD16: 5'-TTCGAAAAGCTACTGTCCAAAAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:25487945
   
wt amplicon (over InsPos) LD17 - LD16
wt amplicon start Chr1:25487945
wt amplicon end Chr1:25489070
wt amplicon size 1126 bp
Locus specific primer LD16: 5'-TTCGAAAAGCTACTGTCCAAAAAT-3'
2nd locus specific primer LD17: 5'-AGAGTGAGCATCAATCAAACACAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11