Genotyping and primer details


Genotyping and primer details for 203G06 - At1g71040
Line ID 203G06
Locus At1g71040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:26797714 of line 203G06 Chr1:26797714
   
Insertion allele amplicon 8474 - 17sm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 17sm: 5'-GAAACCAGTATCGACCAAATCTGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:26798114
   
wt amplicon (over InsPos) 08ti - 17sm
wt amplicon start Chr1:26797346
wt amplicon end Chr1:26798114
wt amplicon size 769 bp
Locus specific primer 17sm: 5'-GAAACCAGTATCGACCAAATCTGT-3'
2nd locus specific primer 08ti: 5'-TAAACATAGCCTGGCTCTTGAGTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11