Genotyping and primer details


Genotyping and primer details for 276D06 - At2g03470
Line ID 276D06
Locus At2g03470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:1046884 of line 276D06 Chr2:1046884
   
Insertion allele amplicon 8474 - KR41
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KR41: 5'-TGCTGAAAGTTAATCGATTGTTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:1047332
   
wt amplicon (over InsPos) 18si - KR41
wt amplicon start Chr2:1046606
wt amplicon end Chr2:1047332
wt amplicon size 727 bp
Locus specific primer KR41: 5'-TGCTGAAAGTTAATCGATTGTTTG-3'
2nd locus specific primer 18si: 5'-AGCCTCTGACTGATCAAGAATCTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11