Genotyping and primer details


Genotyping and primer details for 010D10 - At2g17190
Line ID 010D10
Locus At2g17190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:7480240 of line 010D10 Chr2:7480240
   
Insertion allele amplicon 3144 - 63lf
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 63lf: 5'-AGCCCCACAATATGTTCTAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:7479723
   
Insertion allele amplicon 8474 - KU76
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KU76: 5'-TCAATGGCTGTTGTTGATATGATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:7480860
   
Insertion allele amplicon 3144 - KU77
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer KU77: 5'-TCCTGACCAGTGTGGTAAACATAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:7479874
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11