Genotyping and primer details


Genotyping and primer details for 321D12 - At2g20750
Line ID 321D12
Locus At2g20750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:8941658 of line 321D12 Chr2:8941658
   
Insertion allele amplicon 8474 - 25ns
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 25ns: 5'-CCAACAATTCTTTCTCACTTCTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:8941107
   
wt amplicon (over InsPos) 33oj - 25ns
wt amplicon start Chr2:8941107
wt amplicon end Chr2:8942120
wt amplicon size 1014 bp
Locus specific primer 25ns: 5'-CCAACAATTCTTTCTCACTTCTCC-3'
2nd locus specific primer 33oj: 5'-GTCTCCTTCACCGTCTTCATACTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11