Genotyping and primer details


Genotyping and primer details for 184H01 - At2g23370
Line ID 184H01
Locus At2g23370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:9953141 of line 184H01 Chr2:9953141
   
Insertion allele amplicon 8474 - 05um
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 05um: 5'-ACCATTGAGGCACTAGAACAGATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:9952551
   
wt amplicon (over InsPos) 41vc - 05um
wt amplicon start Chr2:9952551
wt amplicon end Chr2:9953467
wt amplicon size 917 bp
Locus specific primer 05um: 5'-ACCATTGAGGCACTAGAACAGATT-3'
2nd locus specific primer 41vc: 5'-AAGCAATTTTGGTAATCGAAAAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11