Genotyping and primer details


Genotyping and primer details for 379G06 - At2g27840
Line ID 379G06
Locus At2g27840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:11862694 of line 379G06 Chr2:11862694
   
Insertion allele amplicon 8474 - 46wp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 46wp: 5'-ATCTGAGTCGGATTCATCATCTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:11863326
   
Insertion allele amplicon 8474 - FS10
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FS10: 5'-AATCTCAAACTCGTGCCCTAAGTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:11862860
   
Insertion allele amplicon 8474 - HV13
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer HV13: 5'-AACCTTATAAATAAGCCGCTCCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:11861982
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11