Genotyping and primer details


Genotyping and primer details for 193D07 - At2g29670
Line ID 193D07
Locus At2g29670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:12682470 of line 193D07 Chr2:12682470
   
Insertion allele amplicon 8474 - 46xd
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 46xd: 5'-GTCATAATAATCGCATCAGCAGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:12683170
   
Insertion allele amplicon 8474 - AJ20
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AJ20: 5'-CCGAAATTGCATTACGATTTTAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:12681901
   
Insertion allele amplicon 8474 - KA21
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KA21: 5'-CCGAAATTGCATTACGATTTTAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:12681901
   
wt amplicon (over InsPos) AN02 - AJ20
wt amplicon start Chr2:12681901
wt amplicon end Chr2:12682975
wt amplicon size 1075 bp
Locus specific primer AJ20: 5'-CCGAAATTGCATTACGATTTTAGT-3'
2nd locus specific primer AN02: 5'-GTGTTTTAAAGACTCCCCACCATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11