Genotyping and primer details


Genotyping and primer details for 644H11 - At2g30240
Line ID 644H11
Locus At2g30240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:12902751 of line 644H11 Chr2:12902751
   
Insertion allele amplicon 8474 - 29fp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 29fp: 5'-GAGGAAAGATGATCAACCAAACAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:12903115
   
wt amplicon (over InsPos) 06go - 29fp
wt amplicon start Chr2:12902404
wt amplicon end Chr2:12903115
wt amplicon size 712 bp
Locus specific primer 29fp: 5'-GAGGAAAGATGATCAACCAAACAT-3'
2nd locus specific primer 06go: 5'-TGTCCTAAGAAAGACGGTCCTAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11