Genotyping and primer details


Genotyping and primer details for 800B03 - At2g33170
Line ID 800B03
Locus At2g33170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:14057071 of line 800B03 Chr2:14057071
   
Insertion allele amplicon 8474 - EU71
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EU71: 5'-ATGACTCTAACCAGTTTTCTCGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:14057699
   
wt amplicon (over InsPos) BB09 - EU71
wt amplicon start Chr2:14056699
wt amplicon end Chr2:14057699
wt amplicon size 1001 bp
Locus specific primer EU71: 5'-ATGACTCTAACCAGTTTTCTCGGA-3'
2nd locus specific primer BB09: 5'-ACTCCAAAGCTGTAGATATCGCAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11