Genotyping and primer details


Genotyping and primer details for 692D05 - At2g33240
Line ID 692D05
Locus At2g33240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:14091425 of line 692D05 Chr2:14091425
   
Insertion allele amplicon 8474 - 42bk
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 42bk: 5'-CCTTACCTTGAGCTGTTCATTTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:14090987
   
wt amplicon (over InsPos) 06cu - 42bk
wt amplicon start Chr2:14090987
wt amplicon end Chr2:14092018
wt amplicon size 1032 bp
Locus specific primer 42bk: 5'-CCTTACCTTGAGCTGTTCATTTTC-3'
2nd locus specific primer 06cu: 5'-GCATTTAAAAAGTTGTGTGCTTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11