Genotyping and primer details


Genotyping and primer details for 122B02 - At2g34660
Line ID 122B02
Locus At2g34660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:14609499 of line 122B02 Chr2:14609499
   
Insertion allele amplicon 8474 - 04fp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 04fp: 5'-TCAAATCTTCACTCATCCATCAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:14609766
   
wt amplicon (over InsPos) 12gf - 04fp
wt amplicon start Chr2:14608909
wt amplicon end Chr2:14609766
wt amplicon size 858 bp
Locus specific primer 04fp: 5'-TCAAATCTTCACTCATCCATCAAG-3'
2nd locus specific primer 12gf: 5'-TTGTGTCTGGTCATTATGGGAATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11