Genotyping and primer details


Genotyping and primer details for 749B08 - At2g36870
Line ID 749B08
Locus At2g36870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:15473697 of line 749B08 Chr2:15473697
   
Insertion allele amplicon 8474 - 17fb
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 17fb: 5'-CATAGAATTTTTGGGGACAACATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:15474088
   
wt amplicon (over InsPos) 38gc - 17fb
wt amplicon start Chr2:15473095
wt amplicon end Chr2:15474088
wt amplicon size 994 bp
Locus specific primer 17fb: 5'-CATAGAATTTTTGGGGACAACATT-3'
2nd locus specific primer 38gc: 5'-TAGTCGGCTTTGTATTTACCGTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11