Genotyping and primer details


Genotyping and primer details for 561E03 - At2g37410
Line ID 561E03
Locus At2g37410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:15698647 of line 561E03 Chr2:15698647
   
Insertion allele amplicon 8474 - NG39
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NG39: 5'-TTCTAGCCAACCCTAATAAACCCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:15699299
   
wt amplicon (over InsPos) 16ep - NG39
wt amplicon start Chr2:15698251
wt amplicon end Chr2:15699299
wt amplicon size 1049 bp
Locus specific primer NG39: 5'-TTCTAGCCAACCCTAATAAACCCT-3'
2nd locus specific primer 16ep: 5'-TGATGAAGCTGTATTCTGGTTTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11