Genotyping and primer details


Genotyping and primer details for 098H02 - At2g40280
Line ID 098H02
Locus At2g40280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:16827586 of line 098H02 Chr2:16827586
   
Insertion allele amplicon 8474 - BG01
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BG01: 5'-TTCCAATAACAGAAAGTGTTGCAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:16827242
   
wt amplicon (over InsPos) 03sl - BG01
wt amplicon start Chr2:16827242
wt amplicon end Chr2:16828065
wt amplicon size 824 bp
Locus specific primer BG01: 5'-TTCCAATAACAGAAAGTGTTGCAG-3'
2nd locus specific primer 03sl: 5'-AATGGGATCTATGTAAAGGTGCAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11