Genotyping and primer details


Genotyping and primer details for 537E10 - At2g41630
Line ID 537E10
Locus At2g41630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17355834 of line 537E10 Chr2:17355834
   
Insertion allele amplicon 8474 - 09ht
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 09ht: 5'-AAGAGGACAAGCCACGAACTATTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17356415
   
wt amplicon (over InsPos) 05ix - 09ht
wt amplicon start Chr2:17355221
wt amplicon end Chr2:17356415
wt amplicon size 1195 bp
Locus specific primer 09ht: 5'-AAGAGGACAAGCCACGAACTATTA-3'
2nd locus specific primer 05ix: 5'-TTAAAAAGCCATCAAAAATGCTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11