Genotyping and primer details


Genotyping and primer details for 301H05 - At2g44430
Line ID 301H05
Locus At2g44430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:18335128 of line 301H05 Chr2:18335128
   
Insertion allele amplicon 8474 - 16uj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 16uj: 5'-CTTCTTTCGTATCATCTTTTTGGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18335650
   
wt amplicon (over InsPos) 29vc - 16uj
wt amplicon start Chr2:18334624
wt amplicon end Chr2:18335650
wt amplicon size 1027 bp
Locus specific primer 16uj: 5'-CTTCTTTCGTATCATCTTTTTGGC-3'
2nd locus specific primer 29vc: 5'-AAATCACAGCCGTTGATTAGTCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11