Genotyping and primer details


Genotyping and primer details for 879A11 - At2g46880
Line ID 879A11
Locus At2g46880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:19266256 of line 879A11 Chr2:19266256
   
Insertion allele amplicon 8474 - 27ey
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 27ey: 5'-AGATTGTAATTACCAAAACCGTCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:19265730
   
wt amplicon (over InsPos) 06fw - 27ey
wt amplicon start Chr2:19265730
wt amplicon end Chr2:19266791
wt amplicon size 1062 bp
Locus specific primer 27ey: 5'-AGATTGTAATTACCAAAACCGTCG-3'
2nd locus specific primer 06fw: 5'-CTTCCTCTGATTTTGTTTCTTTGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11