Genotyping and primer details


Genotyping and primer details for 262E04 - At3g02290
Line ID 262E04
Locus At3g02290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:460342 of line 262E04 Chr3:460342
   
Insertion allele amplicon 8474 - PP29
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PP29: 5'-TTCTCATGAGGAAGTTGAACCTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:459859
   
wt amplicon (over InsPos) 46wn - PP29
wt amplicon start Chr3:459859
wt amplicon end Chr3:460950
wt amplicon size 1092 bp
Locus specific primer PP29: 5'-TTCTCATGAGGAAGTTGAACCTTT-3'
2nd locus specific primer 46wn: 5'-GAATACAACAACAGAGGCATTTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11