Genotyping and primer details


Genotyping and primer details for 600B11 - At3g03810
Line ID 600B11
Locus At3g03810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:974600 of line 600B11 Chr3:974600
   
Insertion allele amplicon 8474 - 34fm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 34fm: 5'-TAAGCCAGGATGATAAGCTAGGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:973979
   
wt amplicon (over InsPos) 07gf - 34fm
wt amplicon start Chr3:973979
wt amplicon end Chr3:975045
wt amplicon size 1067 bp
Locus specific primer 34fm: 5'-TAAGCCAGGATGATAAGCTAGGAA-3'
2nd locus specific primer 07gf: 5'-TTCCAGAGCTTCAAGAAAGTCTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11