Genotyping and primer details


Genotyping and primer details for 790G01 - At3g04770
Line ID 790G01
Locus At3g04770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:1310843 of line 790G01 Chr3:1310843
   
Insertion allele amplicon 8474 - 11hd
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 11hd: 5'-GATTGCTACAATAACCCTAGCAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:1310412
   
wt amplicon (over InsPos) 10ig - 11hd
wt amplicon start Chr3:1310412
wt amplicon end Chr3:1311254
wt amplicon size 843 bp
Locus specific primer 11hd: 5'-GATTGCTACAATAACCCTAGCAGC-3'
2nd locus specific primer 10ig: 5'-GGCAGGACTCAACTTTTATCTTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11