Genotyping and primer details


Genotyping and primer details for 830A02 - At3g04840
Line ID 830A02
Locus At3g04840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:1330418 of line 830A02 Chr3:1330418
   
Insertion allele amplicon 8474 - 80ra
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 80ra: 5'-TCCTCAAATTACTTCGCTGCTTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:1330179
   
wt amplicon (over InsPos) 16si - 80ra
wt amplicon start Chr3:1330179
wt amplicon end Chr3:1330849
wt amplicon size 671 bp
Locus specific primer 80ra: 5'-TCCTCAAATTACTTCGCTGCTTAT-3'
2nd locus specific primer 16si: 5'-AACTACAAGTATTGCAGGGGAATG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11