Genotyping and primer details


Genotyping and primer details for 072E02 - At3g06850
Line ID 072E02
Locus At3g06850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2160617 of line 072E02 Chr3:2160617
   
Insertion allele amplicon 3144 - DM14
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer DM14: 5'-TGAGCTAGTGGCACATCAATTAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2159974
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11