Genotyping and primer details


Genotyping and primer details for 440E08 - At3g07160
Line ID 440E08
Locus At3g07160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2265393 of line 440E08 Chr3:2265393
   
Insertion allele amplicon 8474 - 40xe
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 40xe: 5'-AAAATTATGCTTTCAGAGTCGTGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2266238
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11