Genotyping and primer details


Genotyping and primer details for 410A07 - At3g09630
Line ID 410A07
Locus At3g09630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2955365 of line 410A07 Chr3:2955365
   
Insertion allele amplicon 8474 - 26gp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 26gp: 5'-CAATGTGATGTTGAAGTTGAATCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2954796
   
wt amplicon (over InsPos) 23hs - 26gp
wt amplicon start Chr3:2954796
wt amplicon end Chr3:2955970
wt amplicon size 1175 bp
Locus specific primer 26gp: 5'-CAATGTGATGTTGAAGTTGAATCC-3'
2nd locus specific primer 23hs: 5'-AACAGATACACACCACGTGTCATT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11