Genotyping and primer details


Genotyping and primer details for 044A02 - At3g12080
Line ID 044A02
Locus At3g12080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:3847999 of line 044A02 Chr3:3847999
   
Insertion allele amplicon 3144 - CT40
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer CT40: 5'-TCAAATGTTAGCAAAACTTACCCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3848580
   
Insertion allele amplicon 8474 - HM07
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer HM07: 5'-GTAACCCAGACTAAACCGAAATCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3847618
   
wt amplicon (over InsPos) HM07 - CT40
wt amplicon start Chr3:3847618
wt amplicon end Chr3:3848580
wt amplicon size 963 bp
Locus specific primer CT40: 5'-TCAAATGTTAGCAAAACTTACCCC-3'
2nd locus specific primer HM07: 5'-GTAACCCAGACTAAACCGAAATCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11