Genotyping and primer details


Genotyping and primer details for 021G04 - At3g17710
Line ID 021G04
Locus At3g17710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:6056226 of line 021G04 Chr3:6056226
   
Insertion allele amplicon 8474 - FL31
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FL31: 5'-AGGACCGTATGTAAACACTGGAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6055675
   
Insertion allele amplicon 8474 - PD01
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PD01: 5'-TAGATGCAAGTAGCTCCAGTTTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6067446
   
wt amplicon (over InsPos) FL31 - PD01
wt amplicon start Chr3:6055675
wt amplicon end Chr3:6067446
wt amplicon size 911 bp
Locus specific primer PD01: 5'-TAGATGCAAGTAGCTCCAGTTTTG-3'
2nd locus specific primer FL31: 5'-AGGACCGTATGTAAACACTGGAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11