Genotyping and primer details


Genotyping and primer details for 156D11 - At3g19670
Line ID 156D11
Locus At3g19670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:6835601 of line 156D11 Chr3:6835601
   
Insertion allele amplicon 8474 - 04ah
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 04ah: 5'-AAGCAAGAGATTGGTTCATTGAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6835045
   
Insertion allele amplicon 8474 - BH69
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BH69: 5'-TTCTCAGAACTTTCAATGTGTTGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6836132
   
wt amplicon (over InsPos) 04ah - BH69
wt amplicon start Chr3:6835045
wt amplicon end Chr3:6836132
wt amplicon size 1088 bp
Locus specific primer BH69: 5'-TTCTCAGAACTTTCAATGTGTTGG-3'
2nd locus specific primer 04ah: 5'-AAGCAAGAGATTGGTTCATTGAGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11