Genotyping and primer details


Genotyping and primer details for 683E11 - At3g20830
Line ID 683E11
Locus At3g20830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:7285062 of line 683E11 Chr3:7285062
   
Insertion allele amplicon 8474 - AB64
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AB64: 5'-TCCAAATCCAATACCCACTAACTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:7284762
   
Insertion allele amplicon 8474 - AR12
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AR12: 5'-TAAGAGCAAGAAGGAGACTTTTCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:7285393
   
wt amplicon (over InsPos) AR12 - AB64
wt amplicon start Chr3:7284762
wt amplicon end Chr3:7285393
wt amplicon size 632 bp
Locus specific primer AB64: 5'-TCCAAATCCAATACCCACTAACTC-3'
2nd locus specific primer AR12: 5'-TAAGAGCAAGAAGGAGACTTTTCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11