Genotyping and primer details


Genotyping and primer details for 065F04 - At3g21250
Line ID 065F04
Locus At3g21250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:7459959 of line 065F04 Chr3:7459959
   
Insertion allele amplicon 8474 - FV35
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FV35: 5'-AGTAGCAGGAAATGCAAAAACAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:7460409
   
Insertion allele amplicon 3144 - FV36
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer FV36: 5'-TTCAAAGAAGAAGCGGATGTTAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:7459400
   
wt amplicon (over InsPos) 05uo - FV36
wt amplicon start Chr3:7459400
wt amplicon end Chr3:7460409
wt amplicon size 1010 bp
Locus specific primer FV36: 5'-TTCAAAGAAGAAGCGGATGTTAAT-3'
2nd locus specific primer 05uo: 5'-AGTAGCAGGAAATGCAAAAACAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11