Genotyping and primer details


Genotyping and primer details for 232A01 - At3g26780
Line ID 232A01
Locus At3g26780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:9849002 of line 232A01 Chr3:9849002
   
Insertion allele amplicon 8474 - MRA1
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MRA1: 5'-CAACCATGAACAAGATAAGCTCAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:9849472
   
wt amplicon (over InsPos) 03qy - MRA1
wt amplicon start Chr3:9848732
wt amplicon end Chr3:9849472
wt amplicon size 741 bp
Locus specific primer MRA1: 5'-CAACCATGAACAAGATAAGCTCAC-3'
2nd locus specific primer 03qy: 5'-CTTTTCACAAAGAAGATGGGTTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11