Genotyping and primer details


Genotyping and primer details for 737D02 - At3g26890
Line ID 737D02
Locus At3g26890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:9909960 of line 737D02 Chr3:9909960
   
Insertion allele amplicon 8474 - 61ad
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 61ad: 5'-GGAGTTCACTGATGATTCGTGTAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:9910275
   
wt amplicon (over InsPos) 16vg - 61ad
wt amplicon start Chr3:9909451
wt amplicon end Chr3:9910275
wt amplicon size 825 bp
Locus specific primer 61ad: 5'-GGAGTTCACTGATGATTCGTGTAG-3'
2nd locus specific primer 16vg: 5'-GTACTTCTCTCCATTGAAAACGCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11