Genotyping and primer details


Genotyping and primer details for 522B11 - At3g26910
Line ID 522B11
Locus At3g26910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:9915250 of line 522B11 Chr3:9915250
   
Insertion allele amplicon 8474 - 07hd
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 07hd: 5'-GATATAGTAGCATCCCCACCGTTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:9915719
   
wt amplicon (over InsPos) 12ix - 07hd
wt amplicon start Chr3:9914892
wt amplicon end Chr3:9915719
wt amplicon size 828 bp
Locus specific primer 07hd: 5'-GATATAGTAGCATCCCCACCGTTA-3'
2nd locus specific primer 12ix: 5'-ATCCTAGATGTCGAGATGACGTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11