Genotyping and primer details


Genotyping and primer details for 500F01 - At3g48010
Line ID 500F01
Locus At3g48010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:17721410 of line 500F01 Chr3:17721410
   
Insertion allele amplicon CR3S - 32vp
T-DNA primer CR3S: 5'-TTGAGCATATAAGAAACCCTTAGT-3'
Locus specific primer 32vp: 5'-CACACCAGAATAACACCACATTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:17722258
   
Insertion allele amplicon 8474 - GA02
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer GA02: 5'-GCACAAAATCATGTAGACGAAGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:17720896
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11