Genotyping and primer details


Genotyping and primer details for 180H10 - At3g48860
Line ID 180H10
Locus At3g48860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:18120330 of line 180H10 Chr3:18120330
   
Insertion allele amplicon 8474 - 09fx
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 09fx: 5'-TGTGGTGTTATAGCTCGCAAATAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18120617
   
wt amplicon (over InsPos) 08gv - 09fx
wt amplicon start Chr3:18119882
wt amplicon end Chr3:18120617
wt amplicon size 736 bp
Locus specific primer 09fx: 5'-TGTGGTGTTATAGCTCGCAAATAC-3'
2nd locus specific primer 08gv: 5'-GTAGTTCTGAAGAGATGCTGGCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11