Genotyping and primer details


Genotyping and primer details for 050E10 - At3g51190
Line ID 050E10
Locus At3g51190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:19017536 of line 050E10 Chr3:19017536
   
Insertion allele amplicon 8474 - CW50
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CW50: 5'-TATTCATGCTTATATTGCAGGCAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:19018178
   
Insertion allele amplicon 3144 - CW51
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer CW51: 5'-AATAATTGGTTTCCCGAAATTACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:19017082
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11