Genotyping and primer details


Genotyping and primer details for 481H08 - At3g52890
Line ID 481H08
Locus At3g52890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:19611703 of line 481H08 Chr3:19611703
   
Insertion allele amplicon 8474 - 44sm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 44sm: 5'-AAGGATTTCAGGTCCATACAACAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:19611345
   
wt amplicon (over InsPos) 03ti - 44sm
wt amplicon start Chr3:19611345
wt amplicon end Chr3:19612036
wt amplicon size 692 bp
Locus specific primer 44sm: 5'-AAGGATTTCAGGTCCATACAACAC-3'
2nd locus specific primer 03ti: 5'-TTGCTTAAAACAACTCGAACTCAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11