Genotyping and primer details


Genotyping and primer details for 016D04 - At3g55000
Line ID 016D04
Locus At3g55000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:20383134 of line 016D04 Chr3:20383134
   
Insertion allele amplicon 3144 - DC48
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer DC48: 5'-CAACTTGCTTTGTTTTCATTTCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20383497
   
Insertion allele amplicon 3144 - FP58
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer FP58: 5'-CTCGCAACCAAGAGAAGAAGTTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20382694
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11