Genotyping and primer details


Genotyping and primer details for 600C07 - At3g58020
Line ID 600C07
Locus At3g58020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21480621 of line 600C07 Chr3:21480621
   
Insertion allele amplicon 8474 - 19qw
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 19qw: 5'-AAAGATCTTGCAATCTCTCTGAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21481012
   
wt amplicon (over InsPos) 03se - 19qw
wt amplicon start Chr3:21480156
wt amplicon end Chr3:21481012
wt amplicon size 857 bp
Locus specific primer 19qw: 5'-AAAGATCTTGCAATCTCTCTGAGC-3'
2nd locus specific primer 03se: 5'-TGCTTCTACAATGAAGGAAGGTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11