Genotyping and primer details


Genotyping and primer details for 890E01 - At3g58120
Line ID 890E01
Locus At3g58120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21521819 of line 890E01 Chr3:21521819
   
Insertion allele amplicon 8474 - HP39
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer HP39: 5'-GGTAATCGTATTCACGACCCTAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21522490
   
wt amplicon (over InsPos) 73rd - HP39
wt amplicon start Chr3:21521354
wt amplicon end Chr3:21522490
wt amplicon size 1137 bp
Locus specific primer HP39: 5'-GGTAATCGTATTCACGACCCTAAA-3'
2nd locus specific primer 73rd: 5'-CATTATTCTCCATCTTCTTGAGGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11