Genotyping and primer details


Genotyping and primer details for 756D02 - At3g58450
Line ID 756D02
Locus At3g58450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21622359 of line 756D02 Chr3:21622359
   
Insertion allele amplicon 8474 - 17mh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 17mh: 5'-AATGCCCTGTATATCTCAAACACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21622945
   
wt amplicon (over InsPos) 08mx - 17mh
wt amplicon start Chr3:21621765
wt amplicon end Chr3:21622945
wt amplicon size 1181 bp
Locus specific primer 17mh: 5'-AATGCCCTGTATATCTCAAACACC-3'
2nd locus specific primer 08mx: 5'-CAAACTCGATTCTAAACCAAAAGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11