Genotyping and primer details


Genotyping and primer details for 092H10 - At3g58480
Line ID 092H10
Locus At3g58480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21629746 of line 092H10 Chr3:21629746
   
Insertion allele amplicon 8474 - 04uj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 04uj: 5'-TAAGGAACTGCTTGAATCTGGTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21629308
   
wt amplicon (over InsPos) 24vg - 04uj
wt amplicon start Chr3:21629308
wt amplicon end Chr3:21630108
wt amplicon size 801 bp
Locus specific primer 04uj: 5'-TAAGGAACTGCTTGAATCTGGTTT-3'
2nd locus specific primer 24vg: 5'-ATAAGCCTTTACCAACCTTAGGGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11