Genotyping and primer details


Genotyping and primer details for 242C01 - At3g61415
Line ID 242C01
Locus At3g61415 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:22723100 of line 242C01 Chr3:22723100
   
Insertion allele amplicon 8474 - 24sm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 24sm: 5'-ATTTGTAAACCCAATTGTGAGGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22722549
   
wt amplicon (over InsPos) 03tp - 24sm
wt amplicon start Chr3:22722549
wt amplicon end Chr3:22723670
wt amplicon size 1122 bp
Locus specific primer 24sm: 5'-ATTTGTAAACCCAATTGTGAGGAG-3'
2nd locus specific primer 03tp: 5'-GAGTCCGTTCCTTCTTCTTAGGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11