Genotyping and primer details


Genotyping and primer details for 791B02 - At3g61720
Line ID 791B02
Locus At3g61720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:22843139 of line 791B02 Chr3:22843139
   
Insertion allele amplicon 8474 - 46vh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 46vh: 5'-TTCGGTCATTGCATCGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22843665
   
wt amplicon (over InsPos) 38vu - 46vh
wt amplicon start Chr3:22842591
wt amplicon end Chr3:22843665
wt amplicon size 1075 bp
Locus specific primer 46vh: 5'-TTCGGTCATTGCATCGGT-3'
2nd locus specific primer 38vu: 5'-CATTGTTATTTTTGCCTTCCAAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11