Genotyping and primer details


Genotyping and primer details for 142F06 - At4g00020
Line ID 142F06
Locus At4g00020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:10363 of line 142F06 Chr4:10363
   
Insertion allele amplicon 8474 - FL18
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FL18: 5'-CGTTCTGCATGTTTAGAAATTTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:10839
   
Insertion allele amplicon 3144 - HP10
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer HP10: 5'-AAGAACTACAGACTTCCCTAGCCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:10040
   
wt amplicon (over InsPos) FL18 - HP10
wt amplicon start Chr4:10040
wt amplicon end Chr4:10839
wt amplicon size 799 bp
Locus specific primer HP10: 5'-AAGAACTACAGACTTCCCTAGCCC-3'
2nd locus specific primer FL18: 5'-CGTTCTGCATGTTTAGAAATTTGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11