Genotyping and primer details


Genotyping and primer details for 125H11 - At4g00260
Line ID 125H11
Locus At4g00260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:116730 of line 125H11 Chr4:116730
   
Insertion allele amplicon 8474 - ME48
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer ME48: 5'-ACTCAAGTATCCACAAGAGCCAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:117336
   
wt amplicon (over InsPos) 13sq - ME48
wt amplicon start Chr4:116368
wt amplicon end Chr4:117336
wt amplicon size 969 bp
Locus specific primer ME48: 5'-ACTCAAGTATCCACAAGAGCCAAT-3'
2nd locus specific primer 13sq: 5'-TAATGAAAAGGGAAAATCATGGAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11