Genotyping and primer details


Genotyping and primer details for 658A06 - At4g10390
Line ID 658A06
Locus At4g10390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:6442467 of line 658A06 Chr4:6442467
   
Insertion allele amplicon 8474 - 88cs
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 88cs: 5'-CTCAATCTTTCTTTGCTTTGCTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:6443215
   
wt amplicon (over InsPos) 14dw - 88cs
wt amplicon start Chr4:6442318
wt amplicon end Chr4:6443215
wt amplicon size 898 bp
Locus specific primer 88cs: 5'-CTCAATCTTTCTTTGCTTTGCTCT-3'
2nd locus specific primer 14dw: 5'-ATAATGAGGGTCTGTGTAACCTGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11