Genotyping and primer details


Genotyping and primer details for 450E06 - At4g19600
Line ID 450E06
Locus At4g19600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:10673736 of line 450E06 Chr4:10673736
   
Insertion allele amplicon 8474 - 22us
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 22us: 5'-TAAAGGACTATTGGGCCATACAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:10674160
   
wt amplicon (over InsPos) 43vc - 22us
wt amplicon start Chr4:10673353
wt amplicon end Chr4:10674160
wt amplicon size 808 bp
Locus specific primer 22us: 5'-TAAAGGACTATTGGGCCATACAAC-3'
2nd locus specific primer 43vc: 5'-ATTCCTGTGCTTTCAGATTCCTAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11